|
|
|
| Registros recuperados: 992 | |
|
|
| MOZDARANI,HOSSEIN; MORADI,SHABNAM Z. |
| This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration... |
| Tipo: Journal article |
Palavras-chave: Cryopreservation; Vitrification; Mouse embryo; Viability; Chromosome abnormalities. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004 |
| |
|
|
| DE LA MAZA,MP; BRAVO,A; LEIVA,L; GATTÁS,V; PETERMANN,M; GARRIDO,F; BUNOUT,D; HIRSCH,S; BARRERA,G; FERNÁNDEZ,M. |
| Introduction: Advanced glycoxidation end-products (AGEs) are involved in age-related conditions and diabetic complications. Diet intake contributes to their circulating concentrations. Aim: To measure serum and urinary AGEs in non-diabetic volunteers and relate their concentration to body composition, blood chemistry and dietary ingestión. Methods: We studied 41 adult men (31 middle-aged adults and 10 elderly). A nutritional assessment including a dietary recall designed for detection of AGE ingestión (specifically carboxymethyl-lysine(CML)), and anthropometric measurements were performed. Also serum lipoproteins, insulin, glucose, leptin and C reactive protein (CRP). AGEs were measured in serum and urine samples using size exclusion chromatography and... |
| Tipo: Journal article |
Palavras-chave: Advanced glycation end-products; Fluorescent AGEs; Glycoxidation. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200011 |
| |
|
| |
|
|
| GARRICK,MICHAEL D; SINGLETON,STEVEN T; VARGAS,FARIDA; KUO,H-C; ZHAO,LIN; KNÖPFEL,MARTIN; DAVIDSON,TODD; COSTA,MAX; PARADKAR,PRASAD; ROTH,JEROME A; GARRICK,LAURA M. |
| DMT1 _ Divalent Metal (Ion) Transporter 1 or SLC11A2/DCT1/Nramp2 _ transports Fe2+ into the duodenum and out of the endosome during the transferrin cycle. DMT1 also is important in non-transferrin bound iron uptake. It plays similar roles in Mn2+ trafficking. Voltage clamping showed that six other metals evoked currents, but it is unclear if these metals are substrates for DMT1. This report summarizes progress on which metals DMT1 transports, focusing on results from the authors' labs. We recently cloned 1A/+IRE and 2/-IRE DMT1 isoforms to generate HEK293 cell lines that express them in a tetracycline-inducible fashion, then compared induced expression to uninduced expression and to endogenous DMT1 expression. Induced expression increases ~50x over... |
| Tipo: Journal article |
Palavras-chave: Metal ion transport; DCT1; Nramp2 or SLC11A2; Expression assay; Competition between metal ions. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100009 |
| |
|
| |
|
|
| KRAUSKOPF,ERWIN. |
| The purpose of this study was to carry out a bibliometric analysis of the articles published in Chile during 1987-2006 that were categorized into the Plant Science discipline by ISI. The data was extracted from the Web of Science datábase, obtaining a total of 911 articles for analysis. Almost half of the articles were the outcome of an international collaboration, the United States being the country with more collaborative links with Chile within this discipline. A list was made with the 10 most-cited articles and 10 most frequently used journals. The analysis showed that almost 50% of the articles were concentrated in 10 different journals. The number of articles produced each year revealed a steady growth in scientific production since 1987. This is... |
| Tipo: Journal article |
Palavras-chave: Bibliometric; Plant science; Productivity; Epistemometric. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200002 |
| |
|
|
| BAPTISTA,FRANCIELLE; AVEZUM ALVES DE CASTRO-PRADO,MARIALBA. |
| The participation of the recently described uvsZ1 mutation in checkpoint control and the identification of epistatic relations between uvsZ1 mutation and uvsD153 and uvsJ1 mutations are provided. The effect of mutation uvsZ1 in mitotic exchanges into paba-bi (chromosome I) and cho-nic (chromosome VII) genetic intervals has also been evaluated. The mutation uvsZ1 was epistatic with regard to uvsD153 and uvsJ1 mutations, with no involvement with checkpoint control. In contrast to mutations in UvsB and UvsF groups, the uvsZ1 mutation failed to cause any changes in the frequencies of mitotic crossing-over. The distinct phenotypic traits given by mutation uvsZ1 suggest the presence of complex interactions among the different DNA repair pathways. Interaction may... |
| Tipo: Journal article |
Palavras-chave: Checkpoint pathways; DNA repair; Uvs mutations. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300018 |
| |
|
|
| Pan,Cuicui; Gao,Hua; Zheng,Ni; Gao,Qi; Si,Yuanquan; Zhao,Yueran. |
| Abstract Background MiR-320 is downregulated in multiple cancers, including glioma and acts as tumor suppressor through inhibiting tumor cells proliferation and inducing apoptosis. PBX3 (Pre-B cell leukemia homeobox 3), a putative target gene of miR-320, has been reported to be upregulated in various tumors and promote tumor cell growth through regulating MAKP/ERK pathway. This study aimed to verify whether miR-320 influences glioma cells growth through regulating PBX3. Methods Twenty-four human glioma and paired adjacent nontumorous tissues were collected for determination of miR-320 and PBX3 expression using RT-qPCR and western blot assays. Luciferase reporter assay was performed to verify the interaction between miR-320 and its targeting sequence in... |
| Tipo: Journal article |
Palavras-chave: Glioma; Luciferase reporter assay; MiR-320; PBX3. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100224 |
| |
|
| |
|
|
| REYES,ANGÉLICA; LEIVA,ANDREA; CAMBIAZO,VERÓNICA; MÉNDEZ,MARCO A; GONZÁLEZ,MAURICIO. |
| Copper is an essential and toxic trace metal for bacteria and, therefore, must be tightly regulated in the cell. Enterococcus hirae is a broadly studied model for copper homeostasis. The intracellular copper levels in E. hirae are regulated by the cop operon, which is formed by four genes: copA and copB that encode ATPases for influx and efflux of copper, respectively; copZ that encodes a copper chaperone; and copY, a copper responsive repressor. Since the complete genome sequence for E. hirae is not available, it is possible that other genes may encode proteins involved in copper homeostasis. Here, we identified a cop-like operon in nine species of Lactobacillale order with a known genome sequence. All of them always encoded a CopY-like repressor and a... |
| Tipo: Journal article |
Palavras-chave: Cop operon; CopY binding site; Copper homeostasis; Enterococcus hirae; Lactobacillale order. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100010 |
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Sadiq,Anwar Zeb Abdul; Ullah,Farhat; Ayaz,Sajjad Ahmad Muhammad. |
| BACKGROUND: Isodon rugosus is used traditionally in the management of hypertension, rheumatism, tooth-ache and pyrexia. Present study was arranged to investigate I. rugosus for phytoconstituents, phytotoxic and cytotoxic activities to explore its toxicological, pharmacological potentials and to rationalize its ethnomedicinal uses. Briefly, qualitative phytochemical analysis of the plant extracts were carried out for the existence of alkaloids, flavonoids, saponins, oils, glycosides, anthraquinones, terpenoids, sterols and tannins. Plant crude methanolic extract (Ir.Cr), its subsequent fractions; n-hexane (Ir.Hex), chloroform (Ir.Chf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq) and saponins (Ir.Sp) in different concentrations were tested for phytotoxic and... |
| Tipo: Journal article |
Palavras-chave: Isodon rugosus; Cytotoxicity; Phytotoxicity; Brine shrimps. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100051 |
| |
|
|
| Orozco-Ibarra,Marisol; Muñoz-Sánchez,Jorge; Zavala-Medina,Martín E; Pineda,Benjamín; Magaña-Maldonado,Roxana; Vázquez-Contreras,Edgar; Maldonado,Perla D; Pedraza-Chaverri,José; Chánez-Cárdenas,María Elena. |
| BACKGROUND: Aged garlic extract (AGE) and its main constituent S-allylcysteine (SAC) are natural antioxidants with protective effects against cerebral ischemia or cancer, events that involve hypoxia stress. Cobalt chloride (CoCl2) has been used to mimic hypoxic conditions through the stabilization of the α subunit of hypoxia inducible factor (HIF-Ια) and up-regulation of HIF-1a-dependent genes as well as activation of hypoxic conditions such as reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and apoptosis. The present study was designed to assess the effect of AGE and SAC on the CoCl2-chemical hypoxia model in PC12 cells RESULTS: We found that CoCl2 induced the stabilization of HIF-1a and its... |
| Tipo: Journal article |
Palavras-chave: Aged garlic extract; Chemical hypoxia; Cobalt chloride; Hypoxia-inducible factor 1; PC12 cells; S-allylcysteine. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100007 |
| |
|
|
| Vàzquez-Salat,Núria. |
| In recent years scientific literature has seen an increase in publications describing new transgenic applications. Although technically-sound, these promising developments might not necessarily translate into products available to the consumer. This article highlights the impact of external factors on the commercial viability of Genetically Modified (GM) animals in the pharmaceutical and food sectors. Through the division of the production chain into three Policy Domains -Science, Market and Public- I present an overview of the broad range of regulatory and socio-economic components that impacts on the path towards commercialisation of GM animals. To further illustrate the unique combination of forces that influence each application, I provide an in-depth... |
| Tipo: Journal article |
Palavras-chave: Genetically modified organisms; GM animals; Policy domains; Governance; Socio-economic factors. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400002 |
| |
|
| |
|
|
| Chen,Peng; Wang,Yuxia; Yan,Lei; Wang,Yiqing; Li,Suyue; Yan,Xiaojuan; Wang,Ningbo; Liang,Ning; Li,Hongyu. |
| BACKGROUND: The development of clean or novel alternative energy has become a global trend that will shape the future of energy. In the present study, 3 microbial strains with different oxygen requirements, including Clostridium acetobutylicum ATCC 824, Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D, were used to construct a hydrogen production system that was composed of a mixed aerobic-facultative anaerobic-anaerobic consortium. The effects of metal ions, organic acids and carbohydrate substrates on this system were analyzed and compared using electrochemical and kinetic assays. It was then tested using small-scale experiments to evaluate its ability to convert starch in 5 L of organic wastewater into hydrogen. For the one-step... |
| Tipo: Journal article |
Palavras-chave: Renewable Energy; Biohydrogen; Microbial consortium; Hydrogen. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100024 |
| |
|
| |
| Registros recuperados: 992 | |
|
|
|
